Figure 2

Immune reaction after cutaneous adenoviral gene delivery. (A) Transduction control of a Bo-Drum®. Representative exposition of a Bo-Drum® 48 h after transduction of fixed skin sample with 1 × 10¹⁰ IU GFP-encoding adenoviral vector (Ad-GFP) (1: transmitted light image; 2: fluorescent image; 3: overlay). (B) Kinetics of cytokine expression after Bo-Drum® transduction. Time course (3 - 96 h) of cytokine mRNA expression (x-fold in relation to a vehicle control) after ex vivo transduction of human full skin in relation to a vehicle control (PBS injection). Per timepoint, n = 6 samples (from two different patients) were transduced with 1 × 10¹⁰ IU Ad-GFP (* = p < 0,05; ** = p < 0,005). (C) Adenovirus induced immune reaction in vivo. GFP-fluorescence detection of immunocompetent, hairless mice (SKH-1^h/r) at timepoints of 7–42 days after intradermal injection (white arrow) of 10¹⁰ IU Ad-GFP (n = 3 per group). An additional vector application and reapplication of the same vector doses was performed on day 14 and 28 (* = p < 0.05, ** = p < 0.005). (D) Type-I-interferon and cytokine expression in vivo. RT-PCR analysis of type-I-interferon and cytokine expression at timepoints of 1, 6, 24, 48, 72 and 120 h after first application (PBS + AdV) and reapplication (AdV + AdV) of 10¹⁰ IU Ad-GFP (* = p < 0.05, # = p < 0.005; AdV + AdV) of 10¹⁰ IU Ad-GFP in immunocompetent (SKH-1^h/r) (A) and athymic (Foxn-1^nu) (B) mice. Data was presented as mean ± SEM (n = 3 per group/timepoint).