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Table 2 Protein annotation in the grapevine fully developed leaves (V1-V15), cork oak ectomycorrhizal roots (S1-S20) and pine needles (P1-P14) spots excised from 2-DE gels and trypsin-digested

From: A possible approach for gel-based proteomic studies in recalcitrant woody plants

Spot

Protein ID

Annotation

Score

Search engine

Protein score

Sequence of the distinct fragmented peptides (p < 0,05)

V1

8615601

cyclase [Vitis pseudoreticulata]

532

ProteinPilot

14

EFESDYAGFTEDGAR

EVILVESLK

KEFESDYAGFTEDGAR

LDDVPAGMYNVHCLHLR

LPGAEGAPIR

SEAYPSAYGSGSCNVELIPVKR

WLVENTDIK

EFESDYAGFTEDGAR

GPALLVDAPR

LPGAEGAPIR

V2

49388156

putative chlorophyll a/b-binding protein type III precursor [Oryza sativa Japonica Group]

270

ProteinPilot

10.67

FQDWANPGSMGK

QGADRPLWFASK

QSLTYLDGSLPGDYGFDPLGLSDPEGTGGFIEPR

QYFLGLEK

WLAYGEVINGR

RFQDWANPGSMGK

LKEVKNGR

QGADRPLWFASK

QYFLGLEK

RFQDWANPGSMGK

WLAYGEVINGR

V3

225446775

oxygen-evolving enhancer protein 2, chloroplastic [Vitis vinifera]

449

ProteinPilot

2.13

SITDYGSPEEFLSK

TNTDFLPYNGEGFK

EFPGQVLR

V4

73647738

ascorbate peroxidase [Vitis pseudoreticulata]

 

ProteinPilot

9.32

ALLSDPAFRPLVEK

EDKPEPPPEGR

NCAPIMLR

SYPTVSEEYKK

TGGPFGTMK

EDKPEPPPEGR

NCAPIMLR

V5

349048

ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial (chloroplast) [Pogostemon cablin]

 

MASCOT

121.49

TFKGPPHGIQVER

V6

225460496

PREDICTED: ATP synthase delta chain, chloroplastic [Vitis vinifera]

 

MASCOT

154.41

LESEHLAQIAK

TAIDPSLVAGFTIR

EIAKEFELVYNR

V7

225461287

PREDICTED: cytochrome b6-f complex iron-sulfur subunit, chloroplastic isoform 1 [Vitis vinifera]

 

MASCOT

234.97

GDPTYLVVENDK

DALGNDVIADEWLK

FICPCHGSQYNNQGR

V8

22797822

ATP synthase epsilon subunit [Vitis vinifera]

 

MASCOT

258.01

TRVEAINVTS

QIIEANLALR

IGNNEITVLVNDAEK

LNDQWLTMALMGGFAR

V9

359475330

PREDICTED: glycine-rich RNA-binding protein GRP1A-like [Vitis vinifera]

 

MASCOT

313.75

DRGYGDGGSR

NITVNEAQSR

AFSQFGEILESK

GGGGGYGGGGGGYGGGSR

GFGFVTFSSEQSMR

CFVGGLAWATDDQSLER

V10

225468761

oxygen-evolving enhancer protein 1, chloroplastic [Vitis vinifera]

610

MASCOT

984.17

VPFLFTIK

RLTYDEIQSK

FGGEFLVPSYR

FCLEPTSFTVK

KFCLEPTSFTVK

DGIDYAAVTVQLPGGER

GTGTANQCPTIDGGVDSFAFK

FEEKDGIDYAAVTVQLPGGER

SKPETGEVIGVFESIQPSDTDLGAK

V11

225459768

plastocyanin, chloroplastic isoform 1 [Vitis vinifera]

331

MASCOT

453.18

GTYSFYCSPHQGAGMVGK

ISMSEEDLLNAPGEVYSVTLTEK

NNAGFPHNVVFDEDEVPSGVDVSK

V12

30687535

Quinone reductase family protein [Arabidopsis thaliana]

393

MASCOT

62.96

AFLDATGGLWR

V13

225456238

PREDICTED: glutamine synthetase cytosolic isozyme 1[Vitis vinifera]

 

MASCOT

183.79

VIVEYIWVGGSGMDLR

GNNILVMCDTYTPAGEPIPTNKR

V14

359473178

Quinone oxidoreductase-like protein At1g23740, chloroplastic-like [Vitis vinifera]

612

MASCOT

460.41

VKPVVDPK

LNPYLESGK

KLNPYLESGK

VVAAALNPVDAK

AWVYGDYGGVDVLK

QFGSFAEYTAVEEK

EGGSVVALTGAVTPPGFR

ELKEGDEVYGDINEK

ATDSPLPTVPGYDVAGVVVK

V15

225432496

PREDICTED: glutamine synthetase leaf isozyme, chloroplastic [Vitis vinifera]

 

MASCOT

349.06

DISDAHYK

AAEIFGNKK

EHISAYGEGNER

TISKPVEHPSELPK

HKEHISAYGEGNER

HETANINTFSWGVANR

GGNNILVICDSYTPAGEPIPTNKR

S1

4838443

symbiosis regulated acidic polypeptide SRAP32-3 [Pisolithus tinctorius]

 

Protein Pilot

4

DKLEAKLDKAAGDYIDGVDI

TDVANSLEFASR

S2

160897637

hypothetical protein Daci_2194 [Delftia acidovorans SPH-1]

 

MASCOT

59.58

ERAQSAAAIER

S3

71659717

hypothetical protein [Trypanosoma cruzi strain CL Brener]

 

MASCOT

58.93

KDIAEEVLER

S4

20162432

AF493154_1 32 kDa-cell wall symbiosis regulated acidic polypeptide [Pisolithus microcarpus]

 

MASCOT

80.35

NDPLYSEAEK

S5

71659717

hypothetical protein [Trypanosoma cruzi strain CL Brener]

 

MASCOT

57

KDIAEEVLER

S6

20162434

32 kDa-cell wall symbiosis regulated acidic polypeptide precursor [Pisolithus microcarpus]

358

MASCOT

210.62

ATGDDYAR

NSLEFAAR

FQLAVCSEK

AADKATGDDYAR

S7

390601324

cysteine peroxiredoxin [Punctularia strigosozonata HHB-11173SS5]

391

MASCOT

334.84

NFDEVLR

TVFVIDPK

LTISYPASTGR

VVDSLQLGDKYR

LGSIAPDFEAETTAGPIK

ISTLYDMLDEQDATNR

S8

225461209

PREDICTED: flavoprotein wrbA isoform 1 [Vitis vinifera]

 

MASCOT

383.52

GAASVEGVEAK

KGAASVEGVEAK

AFLDATGGLWR

GGSPYGAGTFAGDGSR

VKGGSPYGAGTFAGDGSR

VYIVYYSMYGHVEK

S9

20097

jgi|Pisti1|20097|gm1.2716_g

 

MASCOT

54.44

NPDIQAPR

S10

218533914

serine proteinase inhibitor [Clitocybe nebularis]

50.1

MASCOT

119.09

AQEWVIR

YRELQDAYTIVK

S11

20097

jgi|Pisti1|20097|gm1.2716_g

 

MASCOT

302.72

VFAVMEGR

LDEPGEIGWIAPTDGSSQIR

RLDEPGEIGWIAPTDGSSQIR

EIPTAPPGQYRPEELYNLAFPLE

S12

218533914

serine proteinase inhibitor [Clitocybe nebularis]

50.1

MASCOT

89.1

AQEWVIR

ELQDAYTIVK

YRELQDAYTIVK

S13

20097

jgi|Pisti1|20097|gm1.2716_g

 

MASCOT

343.22

LDEPGEIGWIAPTDGSSQIR

RLDEPGEIGWIAPTDGSSQIR

EIPTAPPGQYRPEELYNLAFPLE

S14

33323059

major latex protein [Ficus pumila var. awkeotsang]

187

MASCOT

485.65

GIDEHITKA

LREDVPAPDK

EKVEYDDANR

SPPEKYYNIFK

SATLIGVDGDIMQEYK

GQAYHVPNAAPDHIQGVDVHEGDWETHGSVK

S15

3164115

major latex-like protein [Rubus idaeus]

 

MASCOT

68.2

EKVELDDVNK

S16

Q9S1X8

Na(+)/H(+) antiporter NhaA 1/4[Streptomyces coelicolor strain ATCC BAA-471/A3(2)/M145]

 

MASCOT

483.67

NDAYVIAK

EEREEER

GVGWVAPSPENK

VGECTYVISAR

SVTEPPTFNMEK

KSVTEPPTFNMEK

GVGWVAPSPENKEER

S17

375333787

lectin 2 [Agrocybe aegerita]

565

MASCOT

647.19

FLGEATGDGR

FVVDLTGDGR

DFAYSAGGWR

DGFSIQPFVAIK

ADIVGFGDGGVLVSK

SVIDNFTYSAGGWR

FVLNNFGVQQGWQVNK

NTGGGNFSPASLALNDFGYNAGGWR

S18

392590852

phosphoglycerate mutase-like protein [Coniophora puteana]

540

MASCOT

431.54

VYASPEFK

DIGGIGNLPGR

TAQPFFGAIR

LPPTLIEQAR

GPAPEDRDFLR

ADIPLTEFFYR

SVYLSPSSPSYITNMK

S19

160184939

Serine protease inhibitor [Lentinula edodes (Shiitake mushroom)]

58.9

MASCOT

106.97

WCIQYTER

VGDCTYVISAR

S20

1001331

jgi|Pisti1|1001331|fgenesh1_kg.33_#_73_#_Locus10529v3rpkm0.40_PRE

 

MASCOT

205.69

YYINYLIER

WIITFVPQPGR

NNLLYEQVTAPQK

P1

332591479

phosphoglycerate kinase 1 [Pinus pinaster]

 

MASCOT

260.9

AHASTEGVTK

LTELLGVNVVK

ELDYLVGAVSNPK

ADLNVPLDENQNITDDTR

P2

396547

glutamate-ammonia ligase [Pinus sylvestris]

 

MASCOT

134.75

SLSGPVSSVK

VIAEYIWIGGSGMDMR

P3

218155

chloroplastic aldolase [Oryza sativa Japonica Group]

 

MASCOT

129.98

EAAWGLAR

AKANSLAQLGK

LASIGLENTEANR

P4

3415126

phenylcoumaran benzylic ether reductase [Pinus taeda]

 

MASCOT

497.86

VVILGDGNAR

SLAQAGLTAPPR

ILLIGATGYIGR

DKVVILGDGNAR

ASLDLGHPTFLLVR

FFPSEFGNDVDNVHAVEPAK

GDQTNFEIGPAGVEASQLYPDVK

AIEAEGIPYTYVSSNCFAGYFLR

P5

413951269

ferredoxin-NADP reductase, leaf isozyme [Zea mays]

768

MASCOT

388.09

KDNTYVYMCGLK

RLVYTNDQGEIVK

LYSIASSALGDFGDSK

ITGDDAPGETWHMVFSTEGEIPYR

P6

359473184

carbonic anhydrase, chloroplastic-like isoform 2 [Vitis vinifera]

299

MASCOT

109.34

FMVVACADSR

QTAFIEDWIK

P7

359473184

carbonic anhydrase, chloroplastic-like isoform 2 [Vitis vinifera]

299

MASCOT

107.77

FMVVACADSR

QTAFIEDWIK

P8

14719331

putative 3-beta hydroxysteroid dehydrogenase/isomerase protein [Oryza sativa]

496

MASCOT

245.13

MKPGFDPSK

IGGGDDVFVGDIR

AEQYLADSGLPYTIIR

KAEQYLADSGLPYTIIR

P9

116790330

unknown [Picea sitchensis]

 

MASCOT

104.32

TTFLSDSEVK

TTFLSDSEVKR

P10

116782111

unknown [Picea sitchensis]

 

MASCOT

220.45

EYYNISVLTR

YEDNGDTVSNVSVMVIPTDKK

P11

16798638

AF434186_1 Cu-Zn-superoxide dismutase precursor [Pinus pinaster]

 

MASCOT

234.71

LTHGAPEDDVR

KLTHGAPEDDVR

GGHELSLTTGNAGGR

GNSQVEGVVNLSQEDNGPTTVK

P12

2911276

LMW heat shock protein [Fragaria x ananassa]

103

MASCOT

105.95

QPEPQPPQPK

ASMEDGVLTVTVPK

P13

413946843

Putative peptidyl-prolyl cis-trans isomerase family protein [Zea mays]

307

MASCOT

138.86

TFEDENFK

KLESEETNR

IVLGLFGEDVPK

P14

20794

Type III chlorophyll a/b-binding protein [Pinus sylvestris]

259

MASCOT

268.1

LQDYRNPGSMGK

YLGGSGNPAYPGGPLFNPLGFGK

 

YLGGSGNPAYPGGPLFNPLGFGKDEK

  1. (Protein annotations retrieved from NCBI protein database restricted to Viridiplantae, to Vitis , to Agaricomycotina, JGI Pisolithus tinctorius manual and NCBI Blastp).