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Table 1 Protein yields and total number of 2-DE protein spots, from grape leaves, pine needles, and cork oak ectomycorrhizal ECM roots after phenol, ethanol and TCA-acetone extraction protocols

From: A possible approach for gel-based proteomic studies in recalcitrant woody plants

Plant species

Protocol

Protein yield (mg/g FW)a

Total number of spots

Pine

Phenol

5.81 ± 0.46

805

Ethanol

21.88 ± 4.00

392

TCA-acetone

13.86 ± 1.14

657

Grapevine

Phenol

3.78 ± 0.61

532

Ethanol

20.55 ± 1.79

166

TCA-acetone

16.57 ± 1.31

362

Oak

Phenol

0.61 ± 0.14

904

Ethanol

3.57 ± 0.20

111

 

TCA-acetone

2.77 ± 0.14

36

  1. a Mean and standard deviation from three technical replicates.
  2. FW, fresh weight.