Skip to main content

Table 1 Protein yields and total number of 2-DE protein spots, from grape leaves, pine needles, and cork oak ectomycorrhizal ECM roots after phenol, ethanol and TCA-acetone extraction protocols

From: A possible approach for gel-based proteomic studies in recalcitrant woody plants

Plant species Protocol Protein yield (mg/g FW)a Total number of spots
Pine Phenol 5.81 ± 0.46 805
Ethanol 21.88 ± 4.00 392
TCA-acetone 13.86 ± 1.14 657
Grapevine Phenol 3.78 ± 0.61 532
Ethanol 20.55 ± 1.79 166
TCA-acetone 16.57 ± 1.31 362
Oak Phenol 0.61 ± 0.14 904
Ethanol 3.57 ± 0.20 111
  TCA-acetone 2.77 ± 0.14 36
  1. a Mean and standard deviation from three technical replicates.
  2. FW, fresh weight.