Figure 5
![Figure 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F2193-1801-2-207/MediaObjects/40064_2013_Article_281_Fig5_HTML.jpg)
12% SDS PAGE of TCE induced proteins from PM102 isolate purified through immuno-affinity column chromatography. Lane 1- BSA, Lane 2- total crude protein extracted from PM102 cells grown with 0.2% TCE as the sole carbon source, Lane 3- medium molecular weight marker of 97, 66, 43, 29 and 18.4 kDa respectively, Lane 4 – unbound antigen, lanes 5 to13– bound protein fraction. Four distinct protein bands: P1, P2, P3 and P4 were observed after immuno-affinity column purification of 0.2% TCE grown proteins of PM102 isolate.