Successful antibody subtraction shown by Western Blot and corresponding SDS PAGE profile. a. 12% SDS-PAGE showing proteins extracted from the PM102 isolate. Lane1: medium molecular weight marker; Lane2: 0.2% peptone; Lane3: 0.2%TCE + 0.2% peptone; Lane4: 0.2% TCE. b. Western blot with total serum antibody was found to recognise many proteins. c. Western blot with preadsorbed serum antibody recognised a single protein band of 35.14 kDa in TCE lane and TCE + peptone lane. No reaction seen in peptone lane.