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Figure 1

From: Molecular Characterization of camphor utilizing bacterial isolates from refinery sludge and detection of target loci-Cytochrome P-450 cam mono oxygenase (cam C gene) by PCR and gene probe

Figure 1

(a-c) 16S rDNA gene PCR in camphor isolates. Lanes: a- DNA ladder; b –positive control; c- camphor isolate. (d-f)camC gene PCR in camphor isolates. Lanes: d- DNA ladder; e- positive control; f- camphor isolate. (g-i)Eco RV Restriction digestion pattern of the cam C gene PCR amplicon. Lanes: g- DNA ladder; h & i- restriction digestion patterns of the cam C gene of positive control and camphor isolate showing two bands corresponding to 306 and 210 base pairs respectively. (j) Lane shows 1 kb DNA ladder from Gibco-BRL with detailed MW specifications. Note: Gel picture is divided in three segments, showing results of positive control and one test isolate only (representing remaining camphor isolates that are not included in the gel image).

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