Table 2 Sequences of the primers used in the work
From: PepT1 mRNA expression levels in sea bream (Sparus aurata) fed different plant protein sources
Primer | Sequence 5′ – 3′ | Purpose |
|---|---|---|
PepT1-sense1 | GATGACTTCGCCACCACTA | RT-PCR |
PepT1-antisense1 | CGATCAGATGCAGACGGTG | RT-PCR |
PepT1-sense2 | AGCAGGGCTCAAGATGGAC | RT-PCR |
PepT1-antisense2 | ACATCATCGTGCTCATCGTG | RT-PCR |
PepT1_T7promoter | gtaatacgactcactataggg GGAGTGTGGTATTCACA | mRNA std.curve |
PepT1_antisense3 | AGCAGGGCTCAAGATGGAC | mRNA std.curve |
PepT1 - sense | GCTACCCTCTGGCCTTTGG | Real-time |
PepT1 - antisense | ATGGTGGTAGCTCTGATTGTGTTC | Real-time |
Taqman PepT1 | TCCCCGCTGCTCTC | Real-time |