Table 1 Phenotypic identification of biosurfactant producing Halomonas sp BS4 isolated from solar salt works in India in comparison with other Halomonas sp
Characteristics | Halomonas sp-BS4 | H. salina# | H. halophila# | H. elongate# | H. eurihalina# |
|---|---|---|---|---|---|
Colony Colour | Pink | Cream-yellow | Cream | Cream-beige | Cream |
Cell morphology | Short rod | Short rod | Rod | Long Rod | Short Rod |
Motility | + | ND | ND | ND | ND |
Indole | - | ND | ND | ND | ND |
Methyl Red | - | ND | ND | ND | ND |
VP | - | ND | ND | ND | ND |
NaCl range (% W/v) | 0-20 | 2–20 | 2–30 | 0–25 | 3.5–25 |
NaCl optimum (% W/v) | 8.0 | 5.0 | 7.5 | 11.0 | 2.0 |
pH range | 5.0-10.0 | 5.0–10.0 | 5.0–10.0 | 5.0–9.0 | 5.0–10.0 |
Temperature range | 15-45 | 4–45 | 10–45 | 15–45 | 4–45 |
Nitrate reduction | + | + | + | + | + |
Oxidase | - | + | + | - | - |
Hydrolysis: | |||||
1. Gelatin | + | - | - | + | + |
2. Urea | + | + | + | + | + |
3. Tyrosine | + | + | - | - | + |
EPS production | + | + | - | - | + |
Acid from: | |||||
1. D-Glucose | + | - | + | + | - |
2. L- Arabinose | - | - | + | - | + |
3. D-Galactose | - | - | + | - | - |
4. Lactose | - | - | - | + | - |
5. Maltose | + | - | + | + | - |
6. Mannose | - | - | + | - | - |
7. Sucrose | + | - | - | + | - |
8. Trehalose | ND | - | + | + | - |
GC Content (%) | 53 | 60.5 | 59.1 | 60.4 | 66.7 |