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Table 2 Observed m/z a values of the tryptic peptides derived from rcm-LALF 32-51 -E7 and their theoretical values calculated from the deduced sequence

From: Expression, purification and characterization of a recombinant fusion protein based on the human papillomavirus-16 E7 antigen

Observed

m/z

Theoretical

m/z

Charge

Sequence

411.70

411.70

2

2-7b

761.44

761.47

1

8-13

480.25

480.26

1

23-25

1396.20

1396.20

4

26-75

706.83

706.83

2

76-86

751.35

751.34

1

87-92

664.34

664.34

2

93-103

882.46

882.45

3

104-127

1124.60

1124.57

2

103-123

416.22

416.23

1

124-127

456.70

456.71

2

128-134c

  1. a Mass to charge ratio.
  2. b N-terminal peptide.
  3. c C-terminal peptide.