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Table 1 Summary of LALF 32-51 -E7 purification (from dry weight of the biomass (0.75 g/l))

From: Expression, purification and characterization of a recombinant fusion protein based on the human papillomavirus-16 E7 antigen

Step

Volume (mL)

Total protein concentration (mg/mL)

Purity (%)a

Amount of LALF32-51-E7 (mg)

Purification fold

Step yield (%)

Overall yield

(%)

Initial crude extract

30

5.85

18

31.59

1

100

100

Insoluble fraction after cell disruption

30

2.33

32

22.37

1.7

71

71

Solubilization

15

3.07

45

20.72

1.4

93

66

IMAC elution

25

0.75

94

17.63

2

85

56

Desalting

35

0.5

94

16.45

1

93

52

  1. a Determined by densitometry.
  2. The protein concentration was determined by Bio-Rad protein assay (Bradford 1976), using bovine serum albumin as the reference standard.