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Table 3 Summary of purification of endoglucanase from Bacillus sp MTCC 10046

From: Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung

Sample Volume (ml) Total protein Protein conc. (mg/ml) Total Activity (units) Yield (%) Specific activity (unit/mg) Purification (fold)
Crude 70 14.2 mg 0.202 113.6 100 8.0 1
40-80% dialyzed fraction 15 1.48 mg 0.098 68.37 60.2 46.2 5.8
DEAE column E5 6 0.66 mg 0.11 44.9 39.5 68.1 8.5
  1. Unit = release of reducing sugar in μmoles/ml/min.