Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung

SpringerPlus

Table 2 Effect of different carbon sources 1% (W/V) on cellulase production by Bacillus sp after 8 d of incubation at 37°C

Agricultural waste (1% W/V)	CMCase	Avicelase	FPase	Β-glucosidase
Agricultural waste (1% W/V)	(U/mg protein)	(U/mg protein)	(U/mg protein)	(U/mg protein)
Paddy straw	0.111 ± 0.005	0.197 ± 0.008	0.133 ± 0.005	0.111 ± 0.005
Sugarcane baggasse	0.177 ± 0.0152	0.213 ± 0.010	0.170 ± 0.0152	0.119 ± 0.005
Banana seudo stem	0.130 ± 0.005	0.135 ± 0.0152	0.150 ± 0.0152	0.135 ± 0.0152
Groundnut scale	0.07 ± 0.008	0.07 ± 0.008	0.08 ± 0.0115	0.03 ± 0.0057
Coconut fruit fibre	0.153 ± 0.0152	0.153 ± 0.0152	0.186 ± 0.0152	0.108 ± 0.0115
Orange scale	0.54 ± 1.528	0.59 ± 1.527	0.62 ± 0.0115	0.60 ± 0.015
Whatman filter paper	0.52 ± 0.011	0.50 ± 0.011	0.51 ± 0.011	0.52 ± 0.011
Newspaper	0.65 ± 0.577	0.60 ± 0.015	0.70 ± 0.0185	0.72 ± 0.010
Printing paper	0.50 ± 0.011	0.52 ± 0.011	0.48 ± 1.154	0.45 ± 0.010
Foolscape paper	0.45 ± 0.010	0.42 ± 0.0152	0.50 ± 0.011	0.42 ± 0.0152
CMC	0.73 ± 0.02	0.77 ± 1.201	0.84 ± 0.015	0.93 ± 1.527
Avicel	0.26 ± 0.0152	0.45 ± 0.010	0.32 ± 1.527	0.26 ± 0.0152

The results of one-way factorial ANOVA reveals the amount of enzymes synthesized are in different C-sources varied significantly. F value is significant at P < 0.01 level.