Table 1 Yields and RNase activities of various chromatographic fractions
From: First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha
Column | Fractions | Yield (mg) | Specific activity (U/mg) | Recovery of RNase activity (%) | Purification fold |
|---|---|---|---|---|---|
Ammonium sulfate precipitate | 540 | 14.24 | 100 | 1 | |
DEAE-cellulose | D1 | 12.85 | 15.38 | <3 | - |
D2 | 23.7 | 8.15 | <3 | - | |
D3 | 82.36 | 53.21 | 56.99 | 3.74 | |
D4 | 255.7 | 2.79 | <10 | - | |
S-sepharose | D3S1 | 37.5 | - | - | - |
D3S2 | 0.24 | - | - | - | |
D3S3 | 6.78 | 186.07 | 16.41 | 13.07 | |
D3S4 | 34 | - | - | - | |
Superdex 75 | D3S3SU1 | 0.06 | 6746.67 | 5.29 | 473.78 |
D3S3SU2 | 0.697 | - | - | - |