Table 1 Optimal dilutions of sera and recombinant proteins of Mycobacterium bovis for ELISA
From: Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis
ELISA | Optimal dilutions | |
|---|---|---|
Serum | Recombinant proteins (final concentration μg/ml) | |
CFP-10 | 1: 600 | 1: 10,000 (0.1) |
Chimera * | 1: 600 | 1: 5000 (0.2) |
ESAT-6 | 1: 600 | 1: 5000 (0.2) |
Mb0143 | 1: 600 | 1: 10,000 (0.1) |
MPB83 | 1: 600 | 1: 10,000 (0.1) |
PE13 | 1: 600 | 1: 500 (2.0) |
PE5 | 1: 400 | 1: 500 (2.0) |
TB10.4 | 1: 400 | 1: 2000 (0.5) |
TB15.3 | 1: 400 | 1: 5000 (0.2) |