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Table 2 Effect of solvent and detergents on protease activity from Bacillus subtilis strain VV

From: Cow dung: a potential biomass substrate for the production of detergent-stable dehairing protease by alkaliphilic Bacillus subtilis strain VV

Effect of solvent

Effect of detergents

Solvent (1%)

Residual activity (%)

Detergent (1%)

Relative activity (%)

   

after 1 h

after 24 h

n-Butanol

66.3 ± 4

Sun light

113 ± 7

50 ± 4

Toluene

77.2 ± 4

mr. White

84 ± 5

14 ± 1

Methanol

102.2 ± 3

Henko

99 ± 4

30 ± 5

Acetone

80.5 ± 3

Ujala

110 ± 5

19 ± 3

Acetonitrile

99.2 ± 8

Tide+

85 ± 6

81 ± 5

Benzene

82.6 ± 6

Surf excel

93 ± 5

31 ± 4

Ethanol

92.5 ± 6

Aircel

13 ± 2

0

  1. The protease was pre-incubated with solvent and surfactants at pre-determined time and the remaining activity was measured using the standard protease assay. Residual and relative enzyme activity was determined as percentage of control with no additions.