Table 1 Purification summary of extracellular alkaline protease from Bacillus subtilis strain VV
Purification Step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude enzyme | 16200 | 1005 | 16.11 | 100 | 1 |
Ammonium sulphate | 9740 | 246 | 39.59 | 60.12 | 2.45 |
Sephadex G-75 | 2976 | 19.5 | 152.61 | 18.37 | 9.47 |