From: RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane
Transgene present in cDNA | #Lines | #Lines with RT product detected | RT product detected (%) |
---|---|---|---|
Trehalase mRNA specific RT reaction | |||
Negative Control | 22 | 17 | 77 |
TPSP | 22 | 22 | 100 |
pStarling-trehalase | 30 | 11 | 37 |
RNAi cassette 5′UTR/trehalase mRNA specific RT reaction | |||
pStarling-trehalase | 30 | 18 | 60 |