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Table 1 Expression of the trehalase transcript and the pStarling-trehalase vector

From: RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

Transgene present in cDNA

#Lines

#Lines with RT product detected

RT product detected (%)

Trehalase mRNA specific RT reaction

Negative Control

22

17

77

TPSP

22

22

100

pStarling-trehalase

30

11

37

RNAi cassette 5′UTR/trehalase mRNA specific RT reaction

pStarling-trehalase

30

18

60

  1. RT-PCR was performed using primers specific for the mRNA of trehalase and for a portion of the pStarling-trehalase construct containing the 5′ UTR and the trehalase coding sequence. RT products were blotted onto nitrocellulose membranes and screened with a gene specific probe. Expression was non-quantitatively assigned as positive or negative. RT, reverse transcription; UTR, untranslated region.