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Table 1 Effect of the type of an organic solvent on the degree of hydrolysis at 1 d

From: Promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent

Solvent

Degree of hydrolysis (%)

Control

14.0±1.5

t-Butyl alcohol

32.2±2.0

t-Amyl alcohol

31.5±3.0

Diacetone alcohol

20.5±2.5

Hexane

9.2±2.2

Cyclohexane

6.3±1.1

Cyclohexanone

6.1±1.5

Acetone

0.6±0.1

2-Butanone

1.0±0.2

Diisopropyl ether

7.5±1.0

Diethyl ether

0.7±0.1

Acetonitrile

0.7±0.2

  1. The reaction was performed in triplicate by mixing 2 g CLA menthyl ester, 2 g organic solvent, 1 g water, and 1.8×104 U lipase at 30°C for 1 d.
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