Novel variations in the adiponectin gene (ADIPOQ) may affect distribution of oligomeric complexes

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Table 2 Quantification of serum adiponectin and oligomerization

SNV	Subject^a	RIA	ELISA
SNV	Subject^a	Total (μg/ml)	Total (ug/ml)	HMW + MMW (ug/ml)	HMW (ug/ml)	MMW (ug/ml)	LMW (ug/ml)	% HMW
MIS	Index	0.6	1.1	0.4	0.03	0.37	0.7	0.03
	Match – A	11.6	7.5	5.5	2.9	2.6	2	0.39
	Match - B	3.1	2.6	1.7	0.5	1.2	0.9	0.19
P32L	Index	2.3	2.2	1.3	0.6	0.7	0.9	0.27
	Match - A	12.3	6.4	4.9	2.6	2.3	1.5	0.41
	Match - B	12.7	7	5.3	3.4	1.9	1.7	0.49
R55C	Index	1.2	4.3	0.4	0	0.4	3.9	0.00
	Match - A	4.9	3.4	2	0.7	1.3	1.4	0.21
	Match - B	11.1	4.8	3.1	1.9	1.2	1.7	0.40
Y111H	Index	13.6	6	5.1	2.6	2.5	0.9	0.43
Y111H	Match - A	13.2	8.7	7.3	4.3	3	1.4	0.49
	Match - B	12.6	8.3	6.3	3.7	2.6	2	0.45

Total adiponectin (Total APN), high molecular weight adiponectin (HMW APN), medium molecular weight adiponectin (MMW APN), and low molecular weight adiponectin (LMW APN) were measured by ELISA for each SNV in the subject with the variant and a matched and mismatched control. %HMW: the percentage of adiponectin present in the high molecular weight form.
^a “Index” refers to the individual carrying the variation noted. Both Match A and Match B subjects were sequenced to verify that they did not harbor non synonymous SNVs in ADIPOQ. Match- A refers to the individual selected to match the Index individual by age, sex, race, puberty status, BMI and insulin levels. Match – B refers to the individual selected to match the Index by age, sex, race, puberty status, and BMI, but to be as divergent on insulin levels. ^b Percentiles derived from the larger cohort of 2501 children.