Figure 4

FCdR treatment activates p53 pathway. A. Differentially expressed genes of p53 signaling pathway found in high throughput RNA sequencing were confirmed by real time PCR. B. HCT116 wild type cells were treated with indicated amounts of FCdR for 24 h and p53 was analyzed by western blot. C. HCT116 p53^+/+ and p53^−/− cells were verified by western blot. D. p53^−/− cells were treated with 0.5 μM FCdR for 24 h and expression of indicated p53 downstream genes assayed by real time PCR. These genes were not activated with p53 deficiency. E. p53^−/− cells were treated with FCdR for 48 h and cell cycle was analyzed. FCdR was still able to induce G2/M arrest without p53. (* p value < 0.05; ** p value < 0.01).