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Figure 6

From: Complement anaphylatoxins C3a and C5a induce a failing regenerative program in cardiac resident cells. Evidence of a role for cardiac resident stem cells other than cardiomyocyte renewal

Figure 6

NFκB signalling mediates the induction of Tert by C3a and C5a. A) CPCs transduced with a retroviral vector coding for mutant IκBα that blocks NFκB activation or with a control vector and induction of Tert mRNA expression was assessed by RT-qPCR. B) Chromatin immunoprecipitation (CHIP) of the TERT promoter using a p65 antibody. Input (complete extract), IgG (IgG isotipe control), a-p65 (IP of chromatin binding sites using p65 antibody). Two different primers at the NFκB site of Tert promoter were used mTert-NF-κB(1) and mTert-NF-κB(2). ß-actin primers were used as a control.

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