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Figure 2

From: Complement anaphylatoxins C3a and C5a induce a failing regenerative program in cardiac resident cells. Evidence of a role for cardiac resident stem cells other than cardiomyocyte renewal

Figure 2

CPCs isolation and differentiation. A) CPCs are obtained when cardiospheres are expanded as a monolayer culture in fibronectin coated plates. B) mRNA expression of stemness (Oct4, Sox2, Bmi1, Nestin, Rex1, Tert) and early cardiac markers (Tbx3, Gata4). C) Flow cytometry showing the expression of Sca1 c-kit and CD45 in CPCs. D) Quantitative expression analysis of cardiac, endothelial and smooth muscle markers in CPCs exposed to differentiation medium. E) α-Actinin protein upregulation was confirmed by western-blot after 7 and 14 days in differentiation medium (HL-1 cells protein extract was used as a control). F) Quantitative expression analysis of cardiac, endothelial and smooth muscle markers in GFP+ CPCs after co-culture with NRCMs. G) Immunofluorescence showing GFP+ CPCs expressing Tropomyosin (white arrowheads) after 7 days in co-culture with NRCMs.

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