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Table 1 Predicted gel pattern for analysing various sex identification methods (size in bp)

From: Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies

P2/P8

PCR-RFLP

ARMS-PCR (Multiplex with P2/NP/MP primers)

W-specific PCR

  

Bam HI digest of P2/P8 amplicon

Rsa I digest of P2/P8 amplicon

  

P2/ZW common

P2/W-specific

Gyps bengalensis

Female

Male

Female

Male

Female

Male

Female

Male

Female

Male

Female

Male

389(W)

Nil

389

 

327

327

378(P2/NP-W)

 

153(W)

 

263(W)

Nil

383(Z)

383(Z)

283

283

278

 

372(P2/NP-Z)

372(P2/NP-Z)

153(Z)

153(Z)

  
  

100

100

111

 

293(MP/NP-W)

Nil

    
    

56

56

      

Gyps indicus

389(W)

Nil

389

 

330

330

378(P2/NP-W)

 

153(W)

 

263(W)

Nil

386(Z)

386(Z)

286

286

278

 

375(P2/NP-Z)

375(P2/NP-Z)

153(Z)

153(Z)

  
  

100

100

111

 

293(MP/NP-W)

Nil

    
    

56

56

      

Gyps himalayensis

389(W)

Nil 386(Z)

389

 

330

330

378(P2/NP-W)

 

153(W)

 

263(W)

Nil

386(Z)

 

286

286

278

 

375(P2/NP-Z)

375(P2/NP-Z)

153(Z)

153(Z)

  
  

100

100

111

 

293(MP/NP-W)

Nil

    
    

56

56

      

Aegypius monachus

-

380(Z)

-

280

-

324

-

369(P2/NP-Z)

-

153(Z)

-

Nil

   

100

 

56

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