Figure 5

Southern blot hybridization of the T ₀ putative annexin transgenic greengram plants. Leaf genomic DNA was digested with Pst I, separated by electrophoresis on a 0.8% gel, blotted onto Hybond N + membrane and probed with a 941 by annexin sequence. Lane 1: Positive control (pCAMBIA 2301 + annexin digested with Pst I to release 1.5 kb fragment; Lane 2-3: Empty; Lane 4: Negative control; Lane 5-9: T₀ transformants in which events 7-9 were positive.