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Table 3 The proteolytic activity of L. lactis NZ9000 carrying pNZ45subC

From: Production of the Bacillus licheniformis SubC protease using Lactococcus lactis NICE expression system

Dilutions of NZ9700 supernatant used for induction

Proteolytic activity (U/L)

Non induced

0.84 ± 0.1

100 ×

3.0 ± 0.3

1 000 ×

76.1 ± 5.9

10 000 ×

148.5 ± 0.7

20 000 ×

146.9 ± 5.4

40 000 ×

105.0 ± 2.8

60 000 ×

128.1 ± 1.8

100 000 ×

28.5 ± 0.8

200 000 ×

8.9 ± 3.9

  1. Samples were taken in 24 hours after induction.