Table 1 Strains used in this study
Strain/plasmid | Relevant characteristics | Reference |
|---|---|---|
ATCC 10716 | B. licheniformis | Laboratory stock |
NZ9000 L. lactis | MG1363 pepN::nisRK | (Kuipers et al. 1998) |
NZ9700 L. lactis | Nisin producer | (Kuipers et al. 1995) |
pNZ8048 | CmR, inducible expression vector containing the nisA promoter | (Kuipers et al. 1998) |
pNZ8148 | PnisA, CmR; replicon of rolling circle plasmid pSH71, basic NICE vector, derivative of pNZ8048 | NIZO |
pNZ45 | pNZ8048 carrying SP Usp45 under nisA promoter | This study |
pNZ45subC | pNZ45 carrying subC gene | This study |
pNZ48subC | pNZ8148 carrying subC gene | This study |