Figure 5From: Production of the Bacillus licheniformis SubC protease using Lactococcus lactis NICE expression systemThe proteolytic activity of L. lactis carrying pNZ45subC. The cultures were grown in GM17 medium supplemented with 0.5 mM CaCl2 and 5 μg mL-1 of chloramphenicol. The cultures were induced at OD600 0.7. The samples were taken as indicated on the picture: 2, 3, 4 and 24 hours after induction with 10 000 NZ9700 supernatant. Pale gray bars- strain without induction (values below 1). Black bars- control strain without additional nutrients. Dark gray bars- the strain supplemented with an additional nutrients in two hours after induction. All data are mean values of three independent experiments; error bars indicate standard deviation.Back to article page