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Table 3 Evaluation of fermentation medium for GGH production by A. oryzae IIB-6 in submerged fermentation*

From: Kinetics of improved 1,4-alpha-D-glucan glucohydrolase biosynthesis from a newly isolated Aspergillus oryzae IIB-6 and parameter significance analysis by 2-factorial design

Media (g/l)**

Initial pH

Solvent (1-L)

Protein content (μg/ml)

Enzyme activity (U/ml/min)

DCM (g/l)

Mycelial morphology

Cited bibliography

M1

4.6

0.01 N HCl

27.671±0.521

3.553±0.353

5.541±0.532

Fine pellets

Shoji et al. (2007)

M2

5.5

Deionized H2O

63.544±0.998

9.661±0.713

11.612±0.872

Large pellets

Papagianni (2004)

M3

5

Distilled H2O

73.284±1.523

12.623±0.854

12.964±0.985

Mixed pellets

Michelena and Castillo (1984); Malik et al. (2011)

M4

7.2

Phosphate buffer

17.671±0.986

1.852±0.734

3.456±0.634

Intermediate pellets

Singh and Soni (2001)

M5

6.4

Sodium acetate buffer

53.334±0.721

7.171±0.532

8.613±0.998

Fine pellets

Morita et al. (1998); Norouzian et al. (2006)

LSD

  

0.267

2.651

1.723

  
  1. * Incubation was carried out for 72 h with agitation intensity 200 rpm at temperature and pH 30±2°C and 5, respectively.
  2. ** Media composition is given in the methods section. The constiuents of optimized medium (M3) were further designed in our labs at IIB and have previously been well exploited.
  3. ± indicate the standard deviation (S.D) among the three parallel replicates. The values in each set differ significantly at p≤ 0.05.