Kinetics of improved 1,4-alpha-D-glucan glucohydrolase biosynthesis from a newly isolated Aspergillus oryzae IIB-6 and parameter significance analysis by 2-factorial design

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Table 2 Comparison of various kinetic parameters for GGH productivity by A. kawachii (IIB-2) and A. oryzae (IIB-6) at 72 h of fermentation*

Kinetic parameters	Hyper producing fungal isolates
	IIB-2	IIB-6
Specific growth rate
μ (h^-1)	0.212±0.121^ab	0.196±0.023^a
Enzyme production variables
Q_p (U/ml/h)	0.032±0.022^cde	0.058±0.012^a
Y_p/s (U/ml/g)	0.124±0.0122^def	0.308±0.031^ab
Y_p/x (U/ml/g)	1.625±0.051^defg	2.455±0.552^a
q_p (U/g fungal biomass/h)	0.008±0.002^de	0.210±0.032^abc
Substrate consumption variables
Y_x/s (g fungal biomass/g)	1.124±0.23^ab	1.065±0.112^bc
Q_s (g/l/h)	0.212±0.022^bc	0.268±0.032^a
q_s (g/g fungal biomass/h)	0.125±0.012^bcd	0.195±0.051^ab
Q_x (g fungal biomass/l/h)	0.142±0.021^bc	0.216±0.021^a
Least significant difference (LSD)	0.087	0.034
Significance level <p>	S	HS

* Kinetic parameters: μ (h^-1)=specific growth rate, Q_p=U/ml/h, Y_p/s=U/ml/g substrate consumed, Y_p/x=U/ml/g fungal biomass formed, q_p=U/g fungal biomass/h, Y_x/s=g fungal biomass/g substrate utilized, Q_s=g substrate consumed/l/h, q_s=g substrate consumed/g fungal biomass/h, Q_x=g fungal biomass formed/L/h. HS denotes ‘highly significant’ while S stands for ‘significant’ values. LSD represents ‘least significant difference’. <p> is for probability. ± Indicates standard deviation among three parallel replicates. The values designated by different letters in each row differ significantly at p≤0.05.