Table 1 Primers for amplification of the HPV L1 gene and chimeric variants
Primers | 5’ – 3’ |
|---|---|
HPV 6b L1-F | CACCATGTGGCGGCCTCGCGACAGC |
HPV 6b L1-R | TTACCTTTTAGTTTTGGCGCG |
HPV 6b L1 short-R | TTAAACACCTGTACGAATAGAGG |
Eco-HPV 6b L1-F | CGGAATTCATGTGGCGGCCTCGCGACAGC |
HPV 6b L1-55R-LINKER | TGTACCACCACCACCGCTACCACCACCACCGTTAGCCCGTTTTATGGA |
HPV 6b L1 LINKER-56F | GGAGGAGGAGGAAGCGGAGGAGGAGGAAAAACTGTTGTGCCAAAGGTG |
Kpn-HPV 6b L1-R | GGGGTACCTTACCTTTTAGTTTTGGCGCG |
HPV 6b L1-174R-LINKER | TGTACCACCACCACCGCTACCACCACCACCAGGTGTATTAGTACACTG |
HPV 6b L1 LINKER-175F | GGAGGAGGAGGAAGCGGAGGAGGAGGAGTACAGGCTGGTGACTGCCCG |
HPV 6b L1-348R-LINKER | TGTACCACCACCACCGCTACCACCACCACCGGAAGATGTAGTTACGGA |
HPV 6b L1 LINKER-349F | GGAGGAGGAGGAAGCGGAGGAGGAGGAACATACACCAATTCTGATTAT |
LINKER-EGFP-F | GGTGGTGGTGGTAGCGGTGGTGGTGGTACAATGGTGAGCAAGGGCGAG |
EGFP-R-LINKER | TCCTCCTCCTCCGCTTCCTCCTCCTCCCTTGTACAGCTCGTCCATGCC |