Figure 1
Molecular mass determination. (A): SDS-PAGE analysis. Lane 1, PTL purified by DEAE-sepharose chromatography. Lane 2, active fraction on PHE-sepharose chromatography. Lane 3, Molecular mass reference markers(beta-galactosidase (E. coli) (116.0 kDa), BSA(66.2 kDa), ovalbumin (45.0 kDa), lactate dehydrogenase (35.0 kDa), restriction endozyme (25.0 kDa), beta-lactoglobulin (18.4 kDa) and a-lactalbumin (14.4 kDa)). (B): Native molecular mass estimation of the lectin by gel filtration. Standard markers: BSA (67 kDa), Galanthus nivalis agglutinin (48 kDa), soybean trypsin inhibitor (30.2 kDa), trypsin (23.3 kDa) and cytochrome C (12.5 kDa). The molecular weight size markers (◆) and the lectin (○) are indicated. (C): Molecular mass determination by MALDI-TOF.