Fig. 3
From: Impact of adjustable cryogel properties on the performance of prostate cancer cells in 3D
Growth and spheroid formation of LNCaP cells. a Total metabolic activity analysis of epithelial prostate cancer cells LNCaP seeded in different cryogel scaffolds after 21 days. The viability and proliferation of LNCaP cells was evaluated by applying CellTiter-Glo® 3D Viability assay. Fold change in proliferation was calculated by normalizing the cell number at day 21 to that at day 0. b Calculated volume of LNCaP spheroids after 21 days cell culture. The spheroid width and length was determined by the software of the microscope (AxioVision LE64) of the corresponding HE-slices, which results in the spheroid volume. The experiments were done in triplicate. Error bars represent standard faults. c SEM images of LNCaP spheroids on different cryogel matrices after 21 days of culture at ×35 magnification. Scale bar 200 µm