Fig. 4From: Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategyAmplification plots of the lyophilization assay. Fresh positive control showed a Cycle threshold (Ct) of 22, trehalose 0 % Ct of 24, trehalose 2.5 % Ct of 26, trehalose 5 % Ct of 29 and trehalose 10 % Ct of 33Back to article page