From: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer
Protein conc. (mg/ml)
BSA stock (ml)
Water (ml)
Fluorecamine (ml)
Incubation (min)
0
3.000
0.05
30
0.025
0.015
2.985
0.050
0.030
2.970
0.100
0.060
2.940
0.200
0.120
2.880
0.300
0.180
2.820