Table 1 Preparation of several enzymatic assays
From: Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross-linked enzyme aggregate
Activity assay | Assayed with | Reaction mixtures | Incubation |
|---|---|---|---|
Mannanase | 0.5 % (w/v) locust bean gum (LBG) in 50 mM sodium citrate buffer at pH 4.8 | The enzyme preparation (0.2 ml) was added into 1.8 ml of substrate. | At 50 °C in the water bath for 5 min |
Glucanase | 1 % (w/v) carboxymethylcellulose (CMC) in 50 mM sodium citrate buffer at pH 4.8 | The enzyme preparation (1 ml) was added into 1 ml of substrate and 1.0 ml of sodium citrate buffer | At 50 °C in the water bath for 30 min |
Glucosidase | 15 mM cellobiose in 50 mM sodium citrate buffer at pH 4.8 | The enzyme preparation (0.2 ml) was added to 0.2 ml of substrate and 0.2 ml of sodium citrate buffer | At 50 °C in the water bath for 15 min |
Fructosyltransferase | 40 % (w/v) sucrose in 50 mM sodium acetate buffer at pH 5.4 | The enzyme preparation (1.0 ml) was added into 49 ml of substrate | At 50 °C in the water bath for 5 min |