Table 2 Summary of different protein extraction and precipitation approaches
From: Method developments to extract proteins from oil palm chromoplast for proteomic analysis
Extraction | Precipitation | Protein yield | 2DE gel spots | Pellet solubility | Mass spectrometry interference |
|---|---|---|---|---|---|
100Â % phenol | Ammonium acetate/methanol | Good | 114 | Good | No |
100Â % phenol | TCA/acetone | Good | 73 | Difficult | No |
Phenol/SDS | Ammonium acetate/methanol | Best | 150 | Good | Possible |
SDS alone | Ammonium acetate/methanol | Not determined | 134 | Poor | Possible |
TCA/acetone | – | Not determined | Not suitable for 2DE | Poor | No |