Fig. 5From: Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cellsTamoxifen resistant breast cancer cells have increased pyruvate dehydrogenase activity. On three consecutive days, lysates were prepared from identically treated MCF-7L and TamR-MCF-7 cells. Equal amounts of protein were subjected to a pyruvate dehydrogenase (PDH) assay. The average PDH activity expressed as NADH produced per minute per microgram of protein is shown for each assay replicate; error bars indicate one standard deviation (n = 3 biological replicates × 3 instrument replicates). The p value shown is from a two sided unpaired Student t testBack to article page