Fig. 3

Tamoxifen resistant breast cancer cells have increased PDK4 mRNA expression. a cDNA prepared from cells treated identically to the cells used for the qCR arrays was used as template to measure expression of PDK4 mRNA and 18 s rRNA. Each reaction was performed in triplicate, and the average number of copies of PDK4 mRNA (upper panel) and 18s rRNA (lower panel) are shown; error bars indicate one standard deviation (n = 3 instrument replicates). b Triplicate cultures of MCF-7L and TamR-MCF-7 cells were treated with 1 nM mifepristone, 1 nM dexamethasone, or vehicle control (EtOH) for 2 days in serum free media. Total RNA was extracted, and PDK4 and actin expression were measured by qPCR. The average ratio of PDK4 and actin are shown; error bars indicate one standard deviation (n = 3 biological replicates). Statistically difference between the average ratios are indicated by different letters below the chart. c PDK4 and actin mRNA were measured by qPCR in triplicate cultures of LCC-MCF-7 and LCC9 cells cultured and treated as described in (b)