Table 4 Comparison of the index of substitution saturation (ISS) with the critical index of substitution saturation (ISSc) that defines a threshold for significant saturation in the data

ACT

0.0609

0.6383

37.0781

155.0000

0.0000

GAPDH

0.0454

0.6162

36.7266

163.0000

0.0000

ITS

0.0167

0.7042

146.3186

477.0000

0.0000

TUB2

0.0089

0.6636

87.1685

292.0000

0.0000

ITS1-Loops

0.1665

0.9113

13.6810

50.0000

0.0000

ITS2-Loops

0.0425

0.8548

34.1213

59.0000

0.0000

ITS1-Stem

0.0390

0.4671

19.9356

102.0000

0.0000

ITS2-Stem

0.0481

0.4980

16.5953

96.0000

0.0000