Figure 2

Influence of low pH on dihydroethidium fluorescence in synaptosomes. Role of iron and zinc. a) Kinetics of dihydroethidium fluorescence increase after extracellular acidification. HCl down to pH 6.0 was added where indicated. Curves represent 22 independent experiments. b) Influence of desferoxamine and TPEN on DCF fluorescence evoked by pH 6.0. Con − control. DF − synaptosomes were preincubated for 30 min at 37°C with 100 μM of deferoxamine, incubation medium contains also 100 μM of desferoxamine. TPEN - synaptosomes were preincubated for 30 min at 37°C with 5 μM of TPEN, incubation medium contains also 5 μM of TPEN. Bars represent dihydroethidium fluorescence increase within 4 minutes after additions. Data presented are mean values ± SEM of at least 4 experiments. 100% level corresponds to fluorescence increase in response to pH 6.0 without chelators and antioxidants.