Figure 4

Expression profiles of five genes encoding factors of the creatine pathway in four tissues. Relative copy number of GATM, GAMT, SLC6A8, CKB and CKM was measured in kidney, liver, brain and muscle (8 fish per condition) in relation to the reference genes EEF1A1 and RPS5 using qRT-PCR. Import and BORN trout had been acclimated to 8 and 23°C. Values are shown on a logarithmic scale and are means ± SEM. The line indicates an as high expression of the target gene as the expression of the reference genes. Significance levels between strains and temperatures are marked with an asterisk (*, p < 0.05) and hash sign (#, p < 0.1).