Figure 3

DMC reduces cell viability and induces ER stress in GBM cell lines. (A) Cell viability was assessed in a panel of GBM cell lines after 24h exposure to 0–100 μM of DMC using a MTS assay. Error bars represent S.E.M. of three independent experiments. (B) A172 and U87 cells were treated with 0, 25 or 50 μM DMC for 24h after which apoptosis induction was determined using Annexin V/PI using flow cytometry. (C) Treatment of A172 and U87 cells with 0, 25 or 50 μM DMC for 24h resulted in a dose-dependent up-regulation of CHOP and GRP78 proteins levels as showed by Western blotting. β-actin serves as a loading control. Presented data are representative for at least three independent experiments and mean cell viability levels ± S.E.M. are shown.