Figure 4
From: Cloning, expression and purification of functionally active human angiopoietin-like protein 2
Angptl2-GST promotes endothelial cell migration. Upper Panels) Phase contrast images showing HUVECs that have migrated across the polycarbonate membranes (porosity 5 μm) separating the chambers in the transwells under the following conditions: culture media (Control), supplemented with 1 nM Angptl2-GST or 25 nM VEGF. Lower Panel) Histogram showing the number of cells having migrated across membrane. For each experiment, conditions were assayed in duplicate and 5 randomly chosen fields were quantified per condition. Data shown are mean ± SEM; n = 4; *, p < 0.05 versus control.