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Table 5 Box-Behnken design of three independent variables and experimental vs. predicted PGase values

From: Raw agro-industrial orange peel waste as a low cost effective inducer for alkaline polygalacturonase production from Bacillus licheniformis SHG10

Trial#

X1

X7

X9

Y (PGase) (U/mg)c

Expa.

Predb.

1

-1

-1

0

0.85

0.767

2

1

-1

0

2.03

2.052

3

-1

1

0

2.27

2.247

4

1

1

0

1.15

1.232

5

-1

0

-1

1.79

1.708

6

1

0

-1

2.22

2.033

7

-1

0

1

0.88

1.066

8

1

0

1

0.93

1.011

9

0

-1

-1

0.823

0.987

10

0

1

-1

1.17

1.273

11

0

-1

1

0.215

0.111

12

0

1

1

0.65

0.485

13

0

0

0

2.48

2.640

14

0

0

0

2.68

2.640

15

0

0

0

2.76

2.640

  1. aExperimental values.
  2. bPredicted values.
  3. cU/mg: calculated as μg galacturonic acid.min-1.mg-1.
  4. X1: orange peel waste percent, X7: pH of the production medium and X9: incubation temperature.
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