Table 2 Effects of the ATG initiation codon sequence context of EGFP constructs on translational efficiency of EGFP in cultured cells
Context name | EGFP protein (μg/well) (A) | EGFP mRNA (×10−6) (pmol/ng) (B) | rp49 mRNA (×10−7) (pmol/ng) (C) | EGFP/rp49 mRNA (B/C) | Translational efficiency (A/{B/C}) | Fold efficiency (%) |
|---|---|---|---|---|---|---|
NC-EGFP | 1.60 ± 0.5 | 4.31 ± 1.2 | 6.99 ± 0.17 | 6.15 ± 1.5 | 0.28 ± 0.13 | 100 |
PC-EGFP | 6.37 ± 1.7 | 3.97 ± 1.5 | 6.68 ± 0.57 | 5.92 ± 1.9 | 1.20 ± 0.60 | 424 |
7N3P-EGFP | 4.15 ± 0.6 | 3.47 ± 1.3 | 7.00 ± 0.17 | 4.92 ± 1.7 | 0.94 ± 0.41 | 341 |
7P3N-EGFP | 3.54 ± 0.7 | 3.50 ± 1.1 | 6.81 ± 0.23 | 5.14 ± 1.6 | 0.76 ± 0.34 | 274 |
AAT-EGFP | 4.58 ± 1.2 | 4.32 ± 1.2 | 6.89 ± 0.47 | 6.22 ± 1.5 | 0.79 ± 0.34 | 286 |
AGT-EGFP | 2.72 ± 0.7 | 3.43 ± 1.3 | 6.82 ± 0.25 | 5.00 ± 1.7 | 0.61 ± 0.29 | 216 |
CAA-EGFP | 4.04 ± 1.0 | 4.26 ± 1.2 | 6.63 ± 0.14 | 6.43 ± 1.9 | 0.68 ± 0.30 | 248 |
CGA-EGFP | 1.20 ± 0.2 | 3.21 ± 1.4 | 6.93 ± 0.50 | 4.60 ± 1.8 | 0.30 ± 0.14 | 106 |
Ser1-EGFP | 3.17 ± 0.7 | 3.40 ± 1.0 | 6.74 ± 0.31 | 5.03 ± 1.4 | 0.68 ± 0.28 | 248 |
Vert3-EGFP | 2.87 ± 0.7 | 3.67 ± 1.2 | 7.20 ± 0.27 | 5.09 ± 1.6 | 0.62 ± 0.26 | 224 |
Vert9-EGFP | 2.84 ± 0.3 | 3.30 ± 1.5 | 7.02 ± 0.49 | 4.65 ± 1.8 | 0.68 ± 0.27 | 251 |