Figure 2From: Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature Purification of P5βR2 by amylose resin column chromatography. Soluble protein isolated from early and mid log phase cells which were induced at 4°C. CL, cell lysate; FT, unbound protein in the flow through; W, wash; E, 3.5 μg of eluted protein.Back to article page