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Figure 1 | SpringerPlus

Figure 1

From: Resazurin-based assay for screening bacteria for radiation sensitivity

Figure 1

X- and UV-radiation dose rates within the 96 wells of a microtiter plate. (A) The absorbance within each well was determined at 304 nm (A304) in triplicate experiments using a chemical dosimeter and plotted. The rows (letters) and column (numbers) in the graph associate data with individual wells in the microtiter plate. The average dose rate for 160 kV X-rays was determined from (ΔA304)(280 Gy min-1) to be 17.96 Gy/min (sd = 0.02), and plates received a dose of 54 Gy. (B) A492 values for resazurin absorbance (indicating the cellular metabolic activity within each well) were determined from 6 experiments using a bioassay. Plates received a UV radiation dose of 50 J m-2. Bioassay A492 values were averaged and plotted. The mean value over 96 wells was 0.77 (sd = 0.02) A492 units.

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