Figure 1

Electropherograms of RNA. A: RNA extract using Method 1 (LeukoLOCK™ total RNA isolation system); B: RNA extract using Method 2 (Tempus™ blood RNA tube with ABI PRISM® 6100 Nucleic Acid PrepStation); C: RNA extract using Method 3 (TRIzol® reagent). In evaluating RNA using Agilent 2100 Bioanalyzer, RNA with high quality showed distinct 18S and 28S peaks, and RIN > 8.0. In the present study, all samples showed distinct 18 S and 28 S peaks, and RINs were 9.7, 9.3, and 9.8, respectively, indicating that the RNA isolates had high integrity with negligible RNA degradation.