Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease

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Table 1 Comparative efficacy of bacterial culture, ELISA, conventional and qPCR of IS 900 gene in PB and MB sheep tissues

Pathology	Bacterial culture	ELISA	IS900 PCR positive	qPCR positive
Multibacillary	14/23 (60.9%)^Aa	19/19 (100%)^Aa	19/23 (82.6%)^Aa	23/23 (100%)^Ab
Paucibacillary	05/15 (33.3%)^Bab	2/8 (25%)^Ba	02/15 (13.3%)^Bb	15/15 (100%)^Ac
Total sheep tested	19/38 (50%) ^a	21/27 (77.7%) ^a	21/38 (55.2%) ^a	38/38 (100%) ^b

Values bearing different superscripts within a column (capital letter) and row (small letter) differ significantly at 5% level (P < 0.5).