Figure 5From: Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease IS900 gene PCR for the detection of MAP in intestinal and MLN tissues of naturally MAP infected sheep. Lane M-Marker, L 1- Positive control, L 2- AV22 (Int.), L 3 – AV23 (MLN), L4 – AV31(Int.), L5 – AV31(MLN).Back to article page